our subject isn't cool, but he fakes it anyway
Genetic factors may explain the differences in individual sensitivity to the psychosis-inducing effects of cannabis.
www.nature.com/articles/npjschz201525?WT.mc_id=...
читать дальше
In view of the converging data from candidate gene and genome-wide association studies that the D2-AKT1 signaling pathway is relevant for the pathophysiology and outcome of schizophrenia,3 and on the basis of previous association between cannabis-related psychosis and both DRD2 (rs1076560)1 and AKT1 (rs2494732),2 we hypothesized that these polymorphisms interact in increasing the risk of psychosis in cannabis users. We expected the genetic pathway × cannabis use interaction model to better predict the individual’s odds of psychotic disorder than the single candidate gene×cannabis use interaction model.
Participants were recruited as part of the Genetic and Psychosis project (GAP),2 a case–control study, carried out at the Institute of Psychiatry, London. All patients presenting to the Adult services (18–65 years) of the South London and Maudsley Mental Health NHS Foundation Trust, between December 2005 and October 2010, with their first episode of psychosis, were recruited into the study. Over the same time frame, from the area served by the mental health units, we recruited a sample of control subjects, aged 18–65 years, representing the local population in terms of ethnicity and other main sociodemographics according to the appropriate census data (www.statistics.gov.uk/census). Using the same methodology as in ref. 2, only patients with a diagnosis of nonorganic psychosis (International Classification of Diseases, 10: F20–F29 and F30–F33) were included, and control subjects were excluded if they met criteria for a psychotic disorder or if they reported a previous diagnosis of psychotic illness. For a more detailed description of the GAP study methods, (see refs 1, 2).
The data presented here are based on the subset of the whole GAP sample (450 participants (222 first-episode psychosis patients and 228 healthy individuals) with complete information on the following: (i) sociodemographics (age, gender, and self-reported ethnicity); (ii) lifetime use of cannabis, stimulants, tobacco, and alcohol; and (iii) DRD2 rs1076560 and AKT1 rs2494732 genotypes. To confirm self-report of ethnicity, genetic ancestry was derived using a panel of 57 ancestry-informative genetic markers, as performed previously.2 These were genotyped using iPLEX technology developed for the MassArray platform (Sequenom, San Diego, CA, USA). Ancestry scores were derived using the program Structure to implement a model-based (Markov Chain Monte Carlo) clustering algorithm. Having determined the best solution for K (the probable true number of underlying genetic groups) in initial analyses, individuals who scored between 96 and 100% for genetic cluster membership were used to create a three-way ancestral axis on the basis of Black African (N=81), European Caucasian (N=118), and Asian (N=16) ancestry. These reference groups were used to index genetic ancestry for the remaining sample. Further information on the makeup of the marker panel as well as a figure reporting plots of three-way ancestral axis on the basis of Black African, European Caucasian, and Other are available on request. Ninety percent (N=407) of participants had information on both self-reported ethnicity and ancestry markers. The level of overall agreement between self-reported and genetic ethnicities (96%) was reassuringly high.
Multivariable logistic regressions were used to evaluate the main and interaction effects between two measures of cannabis exposure (lifetime use and frequency of use) and DRD2 rs1076560/AKT1 rs2494732 (individuals carrying one or more of each of the two ‘risk’ alleles (DRD2 T and AKT1 C); individuals carrying one or more ‘risk’ alleles of only one of the genes (DRD2 T or AKT1 C); individuals carrying no ‘risk’ alleles) on presence of a psychotic disorder, after adjusting for potential confounders (sociodemographics and other drug use). The interaction model examined the probability of having a psychotic disorder among cannabis users carrying ‘risk’ allele(s) from both (DRD2 T carriers/AKT1 C carriers) or only one of the genes (DRD2 T carriers/AKT1 TT and DRD2 GG/AKT1 C carriers) compared with DRD2 GG/AKT1 TT subjects. Odds ratio (OR) of psychosis among cannabis-naive subjects carrying ‘risk’ allele(s) from both or only one of the genes were also calculated from the estimates provided by the model.
The study was granted ethical approval by the South London and Maudsley and Institute of Psychiatry Local Research Ethics Committee. All cases and control subjects who were included gave informed written consent, signing the consent document, to the publication of data originating from the study.
Results
First-episode psychosis and control subjects differed significantly for some demographic characteristics and in their patterns of drug use. However, the DRD2/AKT1 genotype was not associated with any sociodemographic variable or cannabis use (all P>0.1; Table 1). A multivariable logistic regression adjusting for the modeled potential confounders showed a significant interaction between DRD2 rs1076560/AKT1 rs2494732 genotypes and lifetime cannabis use on probability of suffering from a psychotic disorder. The analysis showed an increasing probability of suffering from a psychotic disorder in cannabis users depending on DRD2/AKT1 (N=450, likelihood ratio test=7.66; P=0.022). When compared with the no ‘risk’ allele group, cannabis users carrying ‘risk’ allele(s) from only one (OR=3.50; 95% confidence interval: 1.14, 10.77) or both the genes (OR=7.30; 95% confidence interval: 1.58, 33.64) showed increased odds of having psychotic disorder. On the contrary, among those who had never used cannabis, carrying ‘risk’ allele(s) from only one (OR=0.29; 95% confidence interval: 0.09, 0.88) or both the genes (OR=0.14; 95% confidence interval: 0.03, 0.63) was associated with lower odds of suffering a psychotic disorder compared with the DRD2 GG/AKT1 TT genotype (Figure 1a). A second multivariable logistic regression adjusting for the potential confounders showed a significant interaction between DRD2/AKT1 and lifetime frequency of cannabis use on risk of psychosis (N=402, likelihood ratio test =11.91; P=0.042). Among both occasional and daily cannabis users, subjects carrying ‘risk’ allele(s) from one or both genes showed increased odds of having psychotic disorder when compared with the no ‘risk’ allele group; however, only among daily cannabis users did the increased odds of psychosis reach significance. In particular, there was a weak association between daily use and psychosis risk in subjects carrying ‘risk’ allele(s) from only one gene (OR=3.47; 95% confidence interval: 0.99, 12.13) but a strong association between daily use and psychosis risk in subjects carrying ‘risk’ allele(s) from both the genes (OR=10.06; 95% confidence interval: 1.83, 55.17). On the contrary, among those who had never used cannabis, carrying ‘risk’ allele(s) from only one (OR=0.29; 95% confidence interval: 0.08, 1.01) or both the genes (OR=0.10; 95% confidence interval: 0.02, 0.55) was associated with lower odds of suffering a psychotic disorder compared with the DRD2 GG/AKT1 TT genotype (Figure 1b).
www.nature.com/articles/npjschz201525?WT.mc_id=...
читать дальшеIn view of the converging data from candidate gene and genome-wide association studies that the D2-AKT1 signaling pathway is relevant for the pathophysiology and outcome of schizophrenia,3 and on the basis of previous association between cannabis-related psychosis and both DRD2 (rs1076560)1 and AKT1 (rs2494732),2 we hypothesized that these polymorphisms interact in increasing the risk of psychosis in cannabis users. We expected the genetic pathway × cannabis use interaction model to better predict the individual’s odds of psychotic disorder than the single candidate gene×cannabis use interaction model.
Participants were recruited as part of the Genetic and Psychosis project (GAP),2 a case–control study, carried out at the Institute of Psychiatry, London. All patients presenting to the Adult services (18–65 years) of the South London and Maudsley Mental Health NHS Foundation Trust, between December 2005 and October 2010, with their first episode of psychosis, were recruited into the study. Over the same time frame, from the area served by the mental health units, we recruited a sample of control subjects, aged 18–65 years, representing the local population in terms of ethnicity and other main sociodemographics according to the appropriate census data (www.statistics.gov.uk/census). Using the same methodology as in ref. 2, only patients with a diagnosis of nonorganic psychosis (International Classification of Diseases, 10: F20–F29 and F30–F33) were included, and control subjects were excluded if they met criteria for a psychotic disorder or if they reported a previous diagnosis of psychotic illness. For a more detailed description of the GAP study methods, (see refs 1, 2).
The data presented here are based on the subset of the whole GAP sample (450 participants (222 first-episode psychosis patients and 228 healthy individuals) with complete information on the following: (i) sociodemographics (age, gender, and self-reported ethnicity); (ii) lifetime use of cannabis, stimulants, tobacco, and alcohol; and (iii) DRD2 rs1076560 and AKT1 rs2494732 genotypes. To confirm self-report of ethnicity, genetic ancestry was derived using a panel of 57 ancestry-informative genetic markers, as performed previously.2 These were genotyped using iPLEX technology developed for the MassArray platform (Sequenom, San Diego, CA, USA). Ancestry scores were derived using the program Structure to implement a model-based (Markov Chain Monte Carlo) clustering algorithm. Having determined the best solution for K (the probable true number of underlying genetic groups) in initial analyses, individuals who scored between 96 and 100% for genetic cluster membership were used to create a three-way ancestral axis on the basis of Black African (N=81), European Caucasian (N=118), and Asian (N=16) ancestry. These reference groups were used to index genetic ancestry for the remaining sample. Further information on the makeup of the marker panel as well as a figure reporting plots of three-way ancestral axis on the basis of Black African, European Caucasian, and Other are available on request. Ninety percent (N=407) of participants had information on both self-reported ethnicity and ancestry markers. The level of overall agreement between self-reported and genetic ethnicities (96%) was reassuringly high.
Multivariable logistic regressions were used to evaluate the main and interaction effects between two measures of cannabis exposure (lifetime use and frequency of use) and DRD2 rs1076560/AKT1 rs2494732 (individuals carrying one or more of each of the two ‘risk’ alleles (DRD2 T and AKT1 C); individuals carrying one or more ‘risk’ alleles of only one of the genes (DRD2 T or AKT1 C); individuals carrying no ‘risk’ alleles) on presence of a psychotic disorder, after adjusting for potential confounders (sociodemographics and other drug use). The interaction model examined the probability of having a psychotic disorder among cannabis users carrying ‘risk’ allele(s) from both (DRD2 T carriers/AKT1 C carriers) or only one of the genes (DRD2 T carriers/AKT1 TT and DRD2 GG/AKT1 C carriers) compared with DRD2 GG/AKT1 TT subjects. Odds ratio (OR) of psychosis among cannabis-naive subjects carrying ‘risk’ allele(s) from both or only one of the genes were also calculated from the estimates provided by the model.
The study was granted ethical approval by the South London and Maudsley and Institute of Psychiatry Local Research Ethics Committee. All cases and control subjects who were included gave informed written consent, signing the consent document, to the publication of data originating from the study.
Results
First-episode psychosis and control subjects differed significantly for some demographic characteristics and in their patterns of drug use. However, the DRD2/AKT1 genotype was not associated with any sociodemographic variable or cannabis use (all P>0.1; Table 1). A multivariable logistic regression adjusting for the modeled potential confounders showed a significant interaction between DRD2 rs1076560/AKT1 rs2494732 genotypes and lifetime cannabis use on probability of suffering from a psychotic disorder. The analysis showed an increasing probability of suffering from a psychotic disorder in cannabis users depending on DRD2/AKT1 (N=450, likelihood ratio test=7.66; P=0.022). When compared with the no ‘risk’ allele group, cannabis users carrying ‘risk’ allele(s) from only one (OR=3.50; 95% confidence interval: 1.14, 10.77) or both the genes (OR=7.30; 95% confidence interval: 1.58, 33.64) showed increased odds of having psychotic disorder. On the contrary, among those who had never used cannabis, carrying ‘risk’ allele(s) from only one (OR=0.29; 95% confidence interval: 0.09, 0.88) or both the genes (OR=0.14; 95% confidence interval: 0.03, 0.63) was associated with lower odds of suffering a psychotic disorder compared with the DRD2 GG/AKT1 TT genotype (Figure 1a). A second multivariable logistic regression adjusting for the potential confounders showed a significant interaction between DRD2/AKT1 and lifetime frequency of cannabis use on risk of psychosis (N=402, likelihood ratio test =11.91; P=0.042). Among both occasional and daily cannabis users, subjects carrying ‘risk’ allele(s) from one or both genes showed increased odds of having psychotic disorder when compared with the no ‘risk’ allele group; however, only among daily cannabis users did the increased odds of psychosis reach significance. In particular, there was a weak association between daily use and psychosis risk in subjects carrying ‘risk’ allele(s) from only one gene (OR=3.47; 95% confidence interval: 0.99, 12.13) but a strong association between daily use and psychosis risk in subjects carrying ‘risk’ allele(s) from both the genes (OR=10.06; 95% confidence interval: 1.83, 55.17). On the contrary, among those who had never used cannabis, carrying ‘risk’ allele(s) from only one (OR=0.29; 95% confidence interval: 0.08, 1.01) or both the genes (OR=0.10; 95% confidence interval: 0.02, 0.55) was associated with lower odds of suffering a psychotic disorder compared with the DRD2 GG/AKT1 TT genotype (Figure 1b).
@темы: yeah, science!, eat your greens!, ну в общем-то использую любую возможность выложить гадость про нелюбимый наркотик